Effects of wild-type and ▵SH3 BCR/ABL on apoptosis and proliferation. (A) Cells were seeded without growth factors and apoptosis was quantitated at 24 (□) and 48 hours (▨) using an in situ apoptosis detection kit. Results represent the mean ± SD of four independent experiments using individual clones. In (B) and (C), cells from individual clones growing in IMDM-CM were plated with or without, respectively, added WEHI-CM in 96-well plates. The number of cells in each well was scored daily. Results are representative of four independent experiments using individual clones (○) vector, (□) WT, (▵) K1172R, and (•) ▵SH3.