Detection of BCR/ABL transcripts in hematopoietic organs of SCID mice inoculated with BMC infected with wild-type or ▵SH3 BCR/ABL. Preirradiated C57BL/6-SCID-SzJ mice were injected with 10⁶ BMC infected with the wild-type (lanes 1 through 5) or ▵SH3 BCR/ABL (lanes 6 through 10) retrovirus. After 4 weeks, mononuclear cells from bone marrow (BMC), spleen (SPL), and peripheral blood (PBL) were harvested and BCR/ABL transcripts were detected by RT-PCR. To control the efficiency of RT-PCR in each sample, 10² 32Dcl3 transfectants expressing the ▵SH3+▵SH2 BCR/ABL mutant were added to each sample before RNA extraction. As an additional control of the RNA preparation, β-actin transcripts were also amplified from each sample.