Fig. 3.
Lyn coimmunoprecipitates with aggregated FcαR. THP-1 cells (7.5 × 106/sample) were activated at 37°C for 5 minutes with 2 mg of NC particles adsorbed with BSA only (lane 1) or huIgA (lane 2). Immunoprecipitation, separation of precipitated proteins by 8% to 12% SDS-PAGE, and antiphosphotyrosine immunoblot analysis of separated proteins were performed as described before (A). The immunoblot was then stripped and reprobed with a rabbit polyclonal anti-Lyn antibody (B).
