In vitro translation of total reticulocyte RNA isolated from peripheral blood samples of (1) a normal β-globin control, (2 and 3) a carrier (IV7, V6) of IVS-II-4,5 (-AG) mutation, (4) a newborn normal β-globin control, and (5) an in vitro translation reaction to which no exogenous RNA was added. The ³⁵S-labeled translation products were resolved in a Triton-acid-urea polyacrylamide gel. The position of α, α°, β, ^Gγ, δ, and^Aγ globins are indicated on the left of the autoradiograph. Abnormal peptide is marked by an arrow.