DNA sequence of mouse LDHA enhancer and promoter. +1 is the transcription start site. Sites 1 and 2 are the HIF-1 binding sites.24,25 M1 is the mutant in which ACGT was replaced with CATG, and M4 is the mutant in which GCAC was replaced with TACA.24 In this report, we use the following terms. LEn, LDHA enhancer (−186∼Aat II fragment); LP, LDHA promoter (Aat II∼Sac I fragment); LEnLP, LDHA enhancer plus promoter (−186∼Sac I fragment); LEn(M1 or M4)LP, mutant derivatives of the −186∼Sac I fragment.