Fig. 9.
![Fig. 9. Effect of HNF-4 on transient expression of reporter constructs in P19 cells. P19 cells were cotransfected with the reporter plasmids shown in the figure and HNF-4 expression plasmid [pLEn(M4)LPHNF-4] to determine if HNF-4 activates the Epo enhancer in P19 cells. In (A), combination of LDHA promoter (LP) and Epo enhancer (En); in (B), combination of human Epo promoter (0.2k) and En. Transient expression of reporter constructs in 21% and 2% oxygen was assayed. The ratio of HNF-4 expression plasmid versus reporter plasmid is shown. Hypoxic inducibility (the ratio of Luc activity in 2% oxygenv 21% oxygen) is indicated. Each value is the mean ± SD of triplicate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/4/10.1182_blood.v91.4.1185/4/blod4040509.jpeg?Expires=1735537229&Signature=KRvQXE7hRMNJMq8inDU9ODQ5K1kxUMmM9hbggDk~Kxm2Va-3KunEeaNdiIAoCM0OoZPtQueH4wyp7VUbq0oX0JObNUaHpZ8CjCNIewxntUKw0Cau35zdNjVhRf-R9N~CD1hE7faLxgTpH5BxSRs4CYYqR8Fo3dY1al43Fy5QoQaD8l3t2NICUS3IxDzL69ExxHFl1~qfbNdjcD6sMJMd7Kib97Iw1ARLrrG5aPU0jfpb7l7G~HGfymydJ9aYWocHj5KY5ZaSIVAwv961RTuws3zCUaOi3vqImBFBRh4D6eodPM8TgsNLZWLoidYmYufOXMl1O~KppOrsV273zUbqxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HNF-4 on transient expression of reporter constructs in P19 cells. P19 cells were cotransfected with the reporter plasmids shown in the figure and HNF-4 expression plasmid [pLEn(M4)LPHNF-4] to determine if HNF-4 activates the Epo enhancer in P19 cells. In (A), combination of LDHA promoter (LP) and Epo enhancer (En); in (B), combination of human Epo promoter (0.2k) and En. Transient expression of reporter constructs in 21% and 2% oxygen was assayed. The ratio of HNF-4 expression plasmid versus reporter plasmid is shown. Hypoxic inducibility (the ratio of Luc activity in 2% oxygenv 21% oxygen) is indicated. Each value is the mean ± SD of triplicate experiments.
