American Society of Hematology

Fig. 1.

$Fig. 1. Incidence of CFU-O, CFU-M, CFU-GM, and CFU-SCF in FACS fractionated cell populations. Flow cytometric histograms of Lin− cells stained for (A) c-kit, (B) Thy1.2, and (C) Sca1 are shown on the left. The dotted lines indicate isotype controls. N and P indicate the negative and positive gates for cell collection. One or 2 × 103cells/mL from each cell fraction were stimulated with CESJ medium, M-CSF, GM-CSF, or SCF + IL-3 + IL-6 for progenitor analysis. Bar graphs on the right (D-F) represent colony formation from each selected cell population. Means and standard deviations (SDs) from duplicate wells of three independent experiments are shown. (▪) CFU-O; (▩) CFU-M; (▧) CFU-GM; (□) CFU-SCF.$

Incidence of CFU-O, CFU-M, CFU-GM, and CFU-SCF in FACS fractionated cell populations. Flow cytometric histograms of Lin⁻ cells stained for (A) c-kit, (B) Thy1.2, and (C) Sca1 are shown on the left. The dotted lines indicate isotype controls. N and P indicate the negative and positive gates for cell collection. One or 2 × 10³cells/mL from each cell fraction were stimulated with CESJ medium, M-CSF, GM-CSF, or SCF + IL-3 + IL-6 for progenitor analysis. Bar graphs on the right (D-F) represent colony formation from each selected cell population. Means and standard deviations (SDs) from duplicate wells of three independent experiments are shown. (▪) CFU-O; (▩) CFU-M; (▧) CFU-GM; (□) CFU-SCF.

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