The phenotype of freshly isolated and cultured CD34⁺⁺CD38⁻Lin⁻ cells. (A) The electronic gate on the Lin⁻ cell population used to define the CD34⁺⁺CD38⁻Lin⁻ cells is shown. (B) The phenotype of CD34⁺⁺CD38⁻Lin⁻ cells cultured for 2 days in the presence of IL-3, IL-6, GM-CSF, KL, and FL, and additional 3 days on the packaging cells in the presence of same cytokine combination is shown. Most of the cells retain the CD34⁺CD38⁻ phenotype.