DGGE of the amplified exon 10 of factor V gene. Lanes 1 and 3, contol with a homozygosity for G1691→A mutation. Lane 2, control with a heterozygosity for G1691→A mutation. Lane 8, normal control. Lanes 9 and 11, homozygous for G1628→A mutation. Lanes 4 through 7, 10, and 12, heterozygous for G1628→A mutation. (Upstream primer, GC40-TCAGG CAGGA ACAAC ACCAT; downstream primer, GGTTA CTTCA AGGAC AAAAT.).