Fig. 4.
![Fig. 4. Polyacrylamide gel electrophoresis following theBstNI restriction enzyme digest of a 240-bp DNA fragment of exon 7 of factor V gene. Lane M, molecular weight marker. Lane 1,BstNI-digested sample from a normal control showing the two DNA fragments, 100 and 140 bp. Lane 2, BstNI-digested sample from a thrombotic patient heterozygous for A1090→G mutation. It shows three DNA fragments: 100, 140, and 240 bp. Lane 3,BstNI-digested sample from a thrombotic patient without the A1090→G mutation. Similar to lane 1, it shows only 2 DNA fragments, 100 and 140 bp. (Upstream primer, [GC-40]-TGTCC TAACT CAGCT GGGAT; downstream primer, gtatg aaccc caaca actca.)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/4/10.1182_blood.v91.4.1135/4/blod4043704.jpeg?Expires=1735539618&Signature=PrOwvYere9uN9P7p~LVPey7Jfh6d6df9bq30uBQs9kOHTqJOVjYvtpxNRbXkdbe1M8EXYumgIiB3tXl4KLx55GTojp9Pja9S2hAuDCAvXYehQeB96KDy92cZfPlvTV3LSu7b45nEjnfsXgYE8sSty16v4f8Q1nB6jmizRl21E7RU2kJljmzVgSaO2mOLKwE42D6PEyAEKhOGVeJqZ2nikEIo3y9I~n9EyIJhBgKUsQwb1cUC~JvbsDkHUm4uqherAs22g1yE48yZXEWcrwhsQrFLG1xpeoaHgEUilEK1MXa9bPNMNoTmxN2NMS1hh6Y0MyRF2VB0nwJ~qLs3oeOatQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Polyacrylamide gel electrophoresis following theBstNI restriction enzyme digest of a 240-bp DNA fragment of exon 7 of factor V gene. Lane M, molecular weight marker. Lane 1,BstNI-digested sample from a normal control showing the two DNA fragments, 100 and 140 bp. Lane 2, BstNI-digested sample from a thrombotic patient heterozygous for A1090→G mutation. It shows three DNA fragments: 100, 140, and 240 bp. Lane 3,BstNI-digested sample from a thrombotic patient without the A1090→G mutation. Similar to lane 1, it shows only 2 DNA fragments, 100 and 140 bp. (Upstream primer, [GC-40]-TGTCC TAACT CAGCT GGGAT; downstream primer, gtatg aaccc caaca actca.)
