Hga I restriction analysis of PCR products. (A) 2% agarose gel electrophoresis of PCR products from genomic DNA in 6 representative samples. Lanes 2 to 5 show heterozygosity for 46C/T. Lane 6 shows homozygosity for 46C. Lane 7 shows homozygosity for 46T. Undigested DNA remains as a faint 369 bp in 46C homozygotes even after a long duration of digestion. A DNA size marker (100bp ladder) was applied in lanes 1 and 8. (B) 5% polyacrylamide gel electrophoresis of RT-PCR products from liver mRNA in three genetic types for the polymorphism. Lanes 1 to 3 and 5 show heterozygosity for 46C/T. Lane 6 shows homozygosity for 46T. Lane 7 shows homozygosity for 46C. 53 bp bands digested by Hga I were barely visible in this study (not shown). A DNA size marker (100bp ladder) was applied in lane 4.