Flow cytometry analysis of canine BM using MoAb 1H6. Hemolyzed whole BM was stained as described in the Materials and Methods. Cells were incubated with propidium iodide before analysis and cells displayed have been electronically isolated from a gate that excludes debri and cells that retained propidium iodide. Staining of control antibody 31A is shown in (A). In (B), staining with the MoAb 2E9 indicates that 1.66% of BM cells are CD34⁺. Analysis using other projections indicated that CD34⁺ cells are small to large in size and have low to low-intermediate side scattering properties.