Serial effects of a single intravenous dose, 25 μg/kg (○) or 250 μg/kg (•) of PEG-rmMGDF administered on day 0 on platelet number in mice. PEG-rmMGDF produced a profound and dose-dependent increase in platelet number. With both PEG-rmMGDF doses, a 2-day delay was observed before platelet number began to increase; and peak values were reached on day 5. Each data point represents the mean ± SEM of platelet number; for the 25 μg/kg dose, n = 4 to 10 mice per time point for the MGDF group and n = 13 for controls, and for the 250 μg/kg dose, n = 5 to 11 mice per time point for the MGDF group and n = 20 for controls. Data from control mice were pooled to represent the day 0 data point.