Confocal scanning laser microscopy images comparing the intracellular distributions of multimerin with von Willebrand factor and P-selectin. Cells were prepared as in Fig 3. The images are overlays of matching 250 nm optical sections (taken through the cellular plane showing the maximal intensity granular labeling for both proteins) and show labeled structures in the cytoplasm of endothelial cells. Multimerin is shown in red, and P-selectin and von Willebrand factor are shown in green (N indicates the cell nuclei). Differences are seen in the distributions of multimerin, compared to von Willebrand factor and P-selectin. Some of the elongated multimerin-labeled granules (open arrowheads) did not contain detectable von Willebrand factor or P-selectin immunolabel. Regions of possible overlap in the distributions of multimerin and von Willebrand factor or P-selectin are indicated (solid arrows).