The costimulatory capacity of blood derived CMRF-44 purified DC compared with CD14⁺ Mo and CD19⁺ B lymphocytes. (a) The flow cytometry labeling of Nycodenz low-density cells is shown before sorting CMRF-44⁺ CD14⁻ DC and CMRF-44⁻ CD14⁺ Mo. (b) Allogeneic T-lymphocyte proliferative responses measured with ³H thymidine, are shown using each FACS purified cell populations as stimulators.77 The allostimulatory capacity of sorted DC, Mo, and B lymphocytes (CD19⁺ sorted) are compared at a ratio of 1 APC to 50 allogeneic T lymphocytes. (c) DC and Mo are compared in their ability to process and present the soluble protein antigens to KLH and tetanus toxoid to autologous T lymphocytes at an APC:T lymphocyte ratio of 1:20.