Fig. 2.
![Fig. 2. Levels of IFNR mRNA expression in HUT78 variants. The levels of IFNR mRNA were assessed by slot blot analysis. Total poly A+ RNA was isolated from HUT78R and HUT78S cells treated with (+) or without (−) IFNα and diluted with 6.25 mol/L formaldehyde/10X SSPE at 65°C. The treated RNA (10 μg) was transferred to nylon filters, UV-cross-linked and probed with human IFNR cDNA probe labeled with 32[P], followed by autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/2/10.1182_blood.v91.2.570/3/blod4024002.jpeg?Expires=1735834155&Signature=DPB1EGKissDwexpF21bNFSVCKbRfFhqDzaoSLMNSd4KIMZ~Sy6LyBZYujPEmE-HLHf3h8zi~SIonk3DCWmKSovUaLxFeZ0GMMGYaiCD22kjlED-PDs6x~oyqGADIoNql~vbucW992G6yBXOuMUsUKmaCTmrJ3-SH99vrd-mM3RM5FtR2w80gDXVyVyhzz-nuSAVsF1LtamRbTAnQZ6Xb1qcBrNWDdawTg7dPWJ11o190SsxDHJ~0KpT1RGHBHEEGXP-mvNNyUuf~Ng4e9~m4jzg4Mw3eMtiOGHyiLl~FqtwVCdQfHYfWOTohVDEdzgUELm92yLGltpUJSBCkgm8xvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Levels of IFNR mRNA expression in HUT78 variants. The levels of IFNR mRNA were assessed by slot blot analysis. Total poly A+ RNA was isolated from HUT78R and HUT78S cells treated with (+) or without (−) IFNα and diluted with 6.25 mol/L formaldehyde/10X SSPE at 65°C. The treated RNA (10 μg) was transferred to nylon filters, UV-cross-linked and probed with human IFNR cDNA probe labeled with 32[P], followed by autoradiography.
