Fig. 1.
![Fig. 1. Synthesis of FVIII WT and mutants in COS-1 cells. WT and mutant expression plasmids were transfected into COS-1 monkey cells. At 60 hours posttransfection, cells were pulse-labeled with [35S]-methionine for 20 minutes and cell extracts (CE) were harvested. Duplicate plates were chased for 4 hours in medium containing excess unlabeled methionine and then CE were harvested. Equal volumes of CE were immunoprecipitated with anti-FVIII antibody and equal aliquots were analyzed by SDS-PAGE. Mock indicates cells that did not receive plasmid DNA. The migration of FVIII in the CE is indicated at the right as FVIII. Molecular weight size markers are shown on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/2/10.1182_blood.v91.2.538/3/blod4023201.jpeg?Expires=1735500812&Signature=rHPuVLX~M9Yupqb3kXsTWzXbE6xncWTi28LxfHC8p378K5MAW4kLep~a9jbbJqzFhItbOFixA6SX2T3Z~VQG3AUunBoQnV4Jk8wZxMMkVpgvzg9aeLsXcLF3ZlDPMa~HMsZkTV1SqAnzaLliE5UxPIkK3L6y2jl4Kc7~~DC~pvjWC2k1wfGBBAYfJfvqgEJXMaWF~dEYs4YQtaWUM-2THHCiI-OzDF-5SG0DDjucwYUucHujW~ychsiTkaEv8X~OD6h24t6Es12bKtCDpvsV6jJB4FGRyd7I9j3l316RotyFrajd7a0iub69M58NvA~BffEo6nvbG-Vfu8~ytYwFfQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Synthesis of FVIII WT and mutants in COS-1 cells. WT and mutant expression plasmids were transfected into COS-1 monkey cells. At 60 hours posttransfection, cells were pulse-labeled with [35S]-methionine for 20 minutes and cell extracts (CE) were harvested. Duplicate plates were chased for 4 hours in medium containing excess unlabeled methionine and then CE were harvested. Equal volumes of CE were immunoprecipitated with anti-FVIII antibody and equal aliquots were analyzed by SDS-PAGE. Mock indicates cells that did not receive plasmid DNA. The migration of FVIII in the CE is indicated at the right as FVIII. Molecular weight size markers are shown on the left.
