Staining pattern of NA(1+2+)SH(−) (dashed lines) and NA(1+2+)SH(+) neutrophils (continuous lines) with a panel of CD16 MoAbs. One representative experiment of three is shown. (A and B) The reactivity of SH(+) neutrophils with anti–pan-FcγRIII MoAbs (CLBFcRgran1 and 3G8) was higher than that of SH(−) neutrophils. (C and D) The reactivity of both types of neutrophils with NA1-FcγRIIIb–specific MoAbs CLBFcRgran11 and MG38 was comparable. (E) Reactivity of NA2-FcγRIIIb–specific MoAb GRM1 was approximately twice as high with SH(+) neutrophils compared with SH(−) neutrophils. (F) NA2-FcγRIIIb–specific MoAb PEN1 showed a reactivity with SH(+) neutrophils that was sixfold higher compared with the reactivity of SH(−) neutrophils.