Phenotype of sorted CD34⁺⁺Lin⁻ UCB cells. Flow cytometric analysis of sorted CD34 PE⁺⁺CD1⁻CD3⁻CD4⁻CD7⁻CD8⁻CD19⁻gpA⁻cells, stained after sorting with biotinylated antibodies recognizing CD38, c-kit, HLA-DR, and CD45RA, shown in a second step by SA-TC. Dot plots shows CD34 PE versus TC staining, gated on live cells. Values in the quadrants indicate the percentage of cells present in the corresponding quadrant. Quadrants were set to include 99.5% of sorted cells stained, with isotypic control antibody in the upper left quadrant. The data shown are representative of four independent experiments.