CD34⁺⁺Lin⁻ UCB cells generate T, NK, and dendritic cells in the FTOC. Flow cytometric analysis of thymocytes recovered from FTOC initiated with freshly sorted CD34⁺⁺Lin⁻ UCB cells. Cells were stained with CD1 PE, CD2 FITC, CD3 PE, CD4 PE, CD8α FITC, CD34 PE, CD38 FITC, CD45 FITC, CD56 PE, HLA-DR FITC, and anti-TCRγδ FITC. Dot plots show staining of live, human cells recovered after 11 (A) and 35 (B) days of culture. The values in the crosses indicate the percentage of cells present in the corresponding quadrant. Quadrants were set to include 99% of the cells stained, with isotypic control antibody in the lower left quadrant, except for the CD4 PE versus HLA-DR FITC dot plot in (A), in which quadrants are set to contain the CD4⁺⁺HLA-DR⁺⁺ dendritic cells in the upper right quadrant. The data shown are representative of eight independent experiments.