American Society of Hematology

Fig. 6.

$Fig. 6. GFP+ transduced CD34++Lin− UCB cells do not downregulate GFP expression during thymic development in FTOC. Histogram (A) shows the GFP expression of transduced CD34++Lin− UCB 48 hours postinfection (in this experiment, 49% GFP+ cells; N = number of cells). The cells were purified in a GFP+ fraction (sort gate R1) and a GFP− fraction (sort gate R2) by cell sorting. The unseparated population (indicated by R3) served as a control. Dot plots show flow cytometric analysis of thymocytes recovered after 21 days of FTOC and stained with CD1 PE for FTOC initiated with the GFP+ fraction (B), the GFP− fraction (C), and the unseparated population (D). Flow cytometric analysis of thymocytes stained with CD56 PE, gated on CD3 TC− cells, after 21 days of FTOC initiated with the unseparated population (E) is also shown. Dot plots show staining versus GFP expression of live, human cells recovered. The values in the crosses indicate the percentage of cells present in the corresponding quadrant. Quadrants were set to include 99.5% of the cells stained, with isotypic control antibody in lower quadrants. The data shown are representative of two experiments.$

GFP⁺ transduced CD34⁺⁺Lin⁻ UCB cells do not downregulate GFP expression during thymic development in FTOC. Histogram (A) shows the GFP expression of transduced CD34⁺⁺Lin⁻ UCB 48 hours postinfection (in this experiment, 49% GFP⁺ cells; N = number of cells). The cells were purified in a GFP⁺ fraction (sort gate R1) and a GFP⁻ fraction (sort gate R2) by cell sorting. The unseparated population (indicated by R3) served as a control. Dot plots show flow cytometric analysis of thymocytes recovered after 21 days of FTOC and stained with CD1 PE for FTOC initiated with the GFP⁺ fraction (B), the GFP⁻ fraction (C), and the unseparated population (D). Flow cytometric analysis of thymocytes stained with CD56 PE, gated on CD3 TC⁻ cells, after 21 days of FTOC initiated with the unseparated population (E) is also shown. Dot plots show staining versus GFP expression of live, human cells recovered. The values in the crosses indicate the percentage of cells present in the corresponding quadrant. Quadrants were set to include 99.5% of the cells stained, with isotypic control antibody in lower quadrants. The data shown are representative of two experiments.

This Feature Is Available To Subscribers Only