Effects of sLex and CGP69669A on leukocyte rolling velocity in TNFα-stimulated mouse cremaster muscle. Velocity distributions are shown for leukocytes rolling in TNFα-stimulated cremaster muscle of control IgG-treated mice (A), and for leukocytes rolling in TNFα-stimulated cremaster of mice treated with (B) sLex (100 mg/kg), (C) CGP69669A (100 mg/kg), (D) RB40.34 (10 μg), (E) RB40.34 (10 μg) + sLex (100 mg/kg), and (F) RB40.34 (10 μg) + CGP69669A (100 mg/kg). Visibly interacting leukocytes were assigned to groups rolling at <1 μm/s, 1 to 3 μm/s, 3 to 10 μm/s, 10 to 30 μm/s, 30 to 100 μm/s, 100 to 300 μm/s, and 300 to 1,000 μm/s. A log scale was selected to allow direct comparison of rolling over a wide range of velocities. Each histogram represents at least 40 individual cell velocities measured in 8 to 10 cremasteric venules from 3 or 4 mice, except for the RB40.34 + CGP69669A–treated group, which represents fewer cells (15 cell velocities in 9 venules from 4 mice), since rolling flux % was reduced by treatment.
