(A) LDI-PCR products from HindIII-digested CEMO-1 DNA. Bands of 3.7 and 4.3 kb were amplified, corresponding to the rearrangements seen on Southern blot. Five microliters of PCR product was analyzed on 0.8% agarose stained with ethidium bromide. (B) DNA sequence analysis of translocation breakpoint from 4.3-kb LDI-PCR product (clone CH4.3). DNA sequence is compared with germline IGHJsequence and BCL9 cDNA sequence. Shaded region denotesJH5 sequence. (C) Restriction map of LDI-PCR clone CH4.3. The breakpoint in CEMO-1 is indicated by an arrow. Probe X665 is indicated by heavy horizontal bar. This probe was used to screen PAC library and for Northern and Southern analysis. Bg, Bgl II;H, HindIII; P, Pst I; X,Xho I.