FISH analysis of ETV6 rearrangements. (A) Case 1 with a t(5;12)(q31;p13); (B) case 2 with a t(6;12;17)(p21;p13;q25), (C) case 3 with a t(7;12)(p15;p13); (D) case 4 with a t(7;12)(p12;p13); (E) case 5 with a t(7;12)(q36;p13); (F) case 6 with a t(12;13)(p13;q12); and (G and H) case 7 with a der(12)t(5;12;?)(p11;p11p13;?). (A through F) arrowheads indicate derivatives of particular t(12)(p13); (G and H) arrows indicate der(5) and der(14) chromosomes, respectively. All green signals result from probes labeled with a bio-16-dUTP, red signals are generated by probes labeled with Texas Red-5-dUTP, yellow signals result from a mixture of bio-16-dUTP–labeled and Texas Red-5-dUTP–labeled probes in ratio 2;1. The probes used for FISH include (A) cosmid 67C6 (green) and a chromosome 12 centromeric probe pBR12 (red); (A inset) cosmid 179A6 (green); (B) cosmid 15A4 (red), library 6 (green) and pBR12 (yellow); (C) mixture of two cosmids 50F4 and 132B11 (green) and pBR12 (red); (D) cosmid 179A6 (green) and pBR12 (red); (E) cosmid 50F4 (green) and pBR12 (red); (F) cosmid 54D5 and library 9 (green), library 13 (red) and a chromosome 12 centromeric probe pBR12 (yellow); (G) cosmid 148B6 (green) and pBR12 (red); (G inset) cosmid 163E7 (green) and library 12 (red); and (H) cosmid 179A6 (green) and pBR12 (red). Note the hybridization of 179A6 and 67C6 to a der(5) and der(12), respectively, in case 1; hybridization of 15A4 with a der(12) and der(17) in case 2; separation of 50F4 and 132B11 cosmids on both derivative chromosomes in case 3; split signal from 179A6 and 50F4 in cases 4 and 5, respectively; split signal from 54D5 on der(12) and der(13), absence of a second 54D5 signal on a del(12)(p12p13), and presence of a chromosome 13 and 9 material on a der(12) in case 6; appearance of only one hybridization signal from cosmids 148B6 (G) and 179A6 (H) on a der(5) and a der(14), respectively, and the presence of additional material on a der(5)t(5;12;?)(p11;p11p13;?) upstream of theETV6-specific cosmid 163E7 (G inset) in case 7.