Fig. 1.
![Fig. 1. Dose-response effect of IL-3 and GM-CSF on DNA synthesis in freshly isolated primary HMØs. Purified MØs were cultured in serum-free macrophage medium alone as a control or in the presence of 5 ng/mL IL-3 and at concentrations of 1, 10, or 20 ng/mL GM-CSF, as indicated. Cells (2.6 × 104) were seeded in each well of a 96-well plate. On different days, as shown in the figure, cells were pulsed with 0.1 μmol/L or 2.5 μCi/mL [3H] thymidine and harvested after 6 hours, and counts were taken. The data presented here are the average of counts from 6 individual wells ± standard error.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/6/10.1182_blood.v91.6.2108/2/blod4061601.jpeg?Expires=1735559050&Signature=E7m~WReg4P3Onx-ePGgwmcF1J0Aen4KQegyNT6DeBKMfkkD879g6q45M7sL7qET2SwFTPLje5-ksUtAl0E1M4DQSPdtdEuYbM8-1uRAXpThUQbJLCtUFzHANiBvTMAZQYA-wbnzW1Mib5AWIOQnYNHJLwOcOpDfqe~AkoH~nS32Kz~MalgVR7oVSVOoebfkxGYmvuHkWxPrY3zO56QxoeSamv7CfSO07Hsnp2nhX7-yyCi8HPf~cUKgqmLbpaRHQlc1uK5r2X~15pvX1-wk9pJWvxn4G16lgt7WpELhL4wzF-u03SnB5~htMGK7aVKjGpNrToxH9eJ0vuZUPLy8SZg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dose-response effect of IL-3 and GM-CSF on DNA synthesis in freshly isolated primary HMØs. Purified MØs were cultured in serum-free macrophage medium alone as a control or in the presence of 5 ng/mL IL-3 and at concentrations of 1, 10, or 20 ng/mL GM-CSF, as indicated. Cells (2.6 × 104) were seeded in each well of a 96-well plate. On different days, as shown in the figure, cells were pulsed with 0.1 μmol/L or 2.5 μCi/mL [3H] thymidine and harvested after 6 hours, and counts were taken. The data presented here are the average of counts from 6 individual wells ± standard error.
