Fig. 4.
Determination of FcγRI and II turnover in retrovirus vector-mediated Nef and Fen-expressing U937 cells. Cells were starved for 1 hour and pulse-labeled with 35S-labeled cysteine/methionine for 30 minutes; cytoplasmic extracts were prepared at the indicated time points and analyzed by immunoprecipitation with anti-FcγRI and II antibodies. As shown, Nef-expressing U937 cells had significantly lower half-lives of FcγRI and II than those of thefen-transduced U937 cells.
