Dendritic phenotype of adherent AC cultured in X-VIVO 15 medium with GM-CSF and IL-13. AC were maintained in X-VIVO 15 clinical-grade culture medium with GM-CSF and IL-13 for 5 days. TNFα was added for 2 additional days. Flow cytometric analysis was performed at day 7. Isotype-matched murine MoAbs were used as negative controls (dotted lines).