Fig. 3.
DC obtained in X-VIVO 15 medium stimulate allogenic T-lymphocyte proliferation. DC were generated in clinical-grade culture medium using 7 days of culture of AC in the presence of GM-CSF and IL-4 or GM-CSF and IL-13. TNFα was added for the last 2 days. After 3 washes and irradiation (30 Gy), they were used in graded numbers as stimulator cells for 1.5 × 105 allogenic T cells. Cell proliferation was evaluated by a 12-hour pulse with 3H-TdR after 5 days of coculture. Data are expressed as the mean ± SD of sextuplet culture wells. 3H-TdR incorporation rates of irradiated DC or purified T cells were less than 600 cpm.
