Clonal analysis by inverse PCR of marked human T-cell and myeloid colonies recovered from mice transplanted with CD34⁺CD38⁻ cells. (A) G418-resistant human CFU-GM was recovered from the marrow of 2/14 mice transplanted with CD34⁺CD38⁻cells subjected to a 72-hour transduction. The clonal diversity of the individual, marked human myeloid colonies was assessed by single-colony inverse PCR. Colonies shown are from the mouse that had 17/240 G418-resistant progenitors (Table 2). (B) Human T-cell and myeloid colonies were grown from mice transplanted with CD34⁺CD38⁻ cells transduced for 7 days with FL (Table 3). Colonies from two of the four mice were shown to contain the neo gene by PCR, then were futher subjected to single-colony inverse PCR. Panels B and C show amplified inverse PCR products from marked T-cell and myeloid colonies obtained from each mouse. T = T lymphoid clones. M = myeloid clones.