Kinetic interaction analysis of Glu-plasminogen binding to TF as determined with a BIAcore optical biosensor. (A) Sensorgrams for interaction of denoted concentrations of Glu-plasminogen to sensor-immobilized TF extracellular domain. Association phase: 3-minute injections of Glu-plasminogen at 30 μL/min. Dissociation phase: 4-minute injections of buffer alone at 100 μL/min. (B) dR/dt plots of association data according to equation 1. Linear fits are shown to two different components of the data, the early component (cpt. 1) and the late component (cpt. 2). (C) Concentration dependence of ks values determined from linear fits of cpts. 1 and 2 to equation 1. (D) Dissociation phase data from 1 μmol/L plasminogen sensorgram plotted according to equation 2.