FVII interaction with TF-plasminogen complex as determined by BIA2000 analysis. Glu-plasminogen was injected, at concentrations of 0.125 μmol/L (sensorgram 3), 0.25 μmol/L (sensorgram 2), and 1.0 μmol/L (sensorgram 1), onto sensor-immobilized soluble TF and the sensor surface then washed as in Fig 1. Then, FVII was injected in buffer containing 4 mmol/L CaCl₂ for 3 minutes at 30 μL/min, followed by a wash in buffer alone containing 4 mmol/L CaCl₂. Sensorgram 4 shows binding of FVII to immobilized TF after preinjection and wash with buffers containing no Glu-plasminogen. Sensorgrams labeled 1 through 4 for FVII are continuations of the same-numbered sensorgrams for Glu-plasminogen. (Inset) Bmax values for FVII bound after prebinding of Glu-plasminogen at the different concentrations. Bmax values are the difference between the maximum observed response for FVII association and the response due to bound Glu-plasminogen just before FVII was injected.