TF stimulates uPA-mediated conversion of plasminogen to plasmin. Glu-plasminogen (3 μmol/L) was incubated with tcuPA (400 nmol/L) and with various concentrations of TF apoprotein for 20 minutes, and the reaction mixture was analyzed using SDS-PAGE under reducing conditions. Lane 1 contains plasminogen alone. Lanes 2 through 4 contain plasminogen, tcuPA, and increasing concentrations of TF (lane 2, 40 nmol/L TF; lane 3, 100 nmol/L TF; and lane 4, 300 nmol/L TF).