Effect of hematocrit level on the inhibition of PL and nucleic acid biosyntheses by E7. Experiments were performed for 4 hours at 37°C with the same infected cell suspensions (5.6% parasitemia) either under the high hematocrit level (13%) conditions described in the Materials and Methods, ie, in 60 μL final volume (5 × 106 parasites) (solid symbols) or at low hematocrit level (1.3%) in a final volume of 300 μL (containing 2.5 × 106 parasites) (open symbols), resulting in a 10-fold difference in hematocrit level. In both cases, the concentrations and the specific activities of the radioactive precursors were similar, ie, 10 μmol/L (3H)choline at 1.92 Ci/mmol, 2 μmol/L (3H)ethanolamine at 2.74 Ci/mmol, and trace concentration of (3H)hypoxanthine at 4 μCi/well. The results are derived from a typical experiment performed in triplicate.
