Fig. 1.
Tyrosine phosphorylation of cellular proteins in HEL-JW cells after VRP stimulation. Serum-starved HEL-JW cells (12 × 106/mL) were stimulated with VRP (125 ng/mL) for the indicated times. TCLs were prepared, fractionated on 10% SDS-PAGE and directly subjected to Western blot analysis with the antiphosphotyrosine antibody 4G10. The phosphorylated proteins present after VRP treatment are indicated by arrows.