Binding of CHO-CD36 cells to the rC1-2 fragment of MC PfEMP1. GST-fusion proteins derived from the MC PfEMP1 sequence were immobilized on petri dishes and incubated with 50 μL of 2 × 10⁶ cells/mL of CHO, CHO-CD36, and CHO-ICAM-1 cells. Results are presented as number of bound cells/mm2 ± STD. (A) CHO-CD36 cells specifically bind to immobilized rC1-2 but not to rB1 and rD1 recombinant proteins from MC PfEMP1, rGA62-5, GST, or to the immobilizing MoAb 141 alone (none). CHO cells or CHO-ICAM-1 cells did not bind to any of the recombinant proteins tested. (B) Concentration-dependent binding of CHO-CD36 cells (•) but not CHO cells (○) to immobilized rC1-2.