Fig. 1.
![Fig. 1. Presence of suPAR in conditioned media of vascular cells. (A) suPAR was captured by immobilized anti-uPAR MoAb-R4 and subsequently detected by measuring the extent of uPA binding using a plasminogen activation assay. Recombinant suPAR was used to generate the standard curve (rate of plasmin formation; Vmax, mOD/min at 405 nm) against which the unknown samples were quantified. (B) The production of suPAR by HVSMC, HUVEC, HL-60, and U937 cells was measured under basal conditions (hatched bars) or after stimulation by PMA (100 ng/mL) (filled bars). Data are shown as ng/18 h/106 cells (mean ± standard error of mean [SEM] of triplicate wells) and similar results were observed in three separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2305/3/blod4073801.jpeg?Expires=1735552260&Signature=kmtx11nTC8Yabr2AoVehugM-1hxzMZtA~7Fdj1VZq9wIP9kBYUsWqEnvc96m4gtacggdM2fXmoSWWq7oeftbCsIE27ShTPUL2Rp1CGfwTvJzpgj9dMAQ6XdxlZKCZYzegXkWi0Fep5xIbRbRVxYtKmu8ep2MisU~eOyOCVmNW~R4dLktB6HiNGxDUKh3qu9qeT3zi6-ilLmvlETZ3exajDVfGGlPcDUWhJqRBw07gxYYYPMpNu3dZ0LHSWI6Tzu2Ab4wTjbc7llXIf-soFkAg89nDhVBA09sg8wTYWmnlQ-TG9L-Ln4oJK6A-Guh6tctnF5Q3xlp~9fcjrUOPVOv5A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Presence of suPAR in conditioned media of vascular cells. (A) suPAR was captured by immobilized anti-uPAR MoAb-R4 and subsequently detected by measuring the extent of uPA binding using a plasminogen activation assay. Recombinant suPAR was used to generate the standard curve (rate of plasmin formation; Vmax, mOD/min at 405 nm) against which the unknown samples were quantified. (B) The production of suPAR by HVSMC, HUVEC, HL-60, and U937 cells was measured under basal conditions (hatched bars) or after stimulation by PMA (100 ng/mL) (filled bars). Data are shown as ng/18 h/106 cells (mean ± standard error of mean [SEM] of triplicate wells) and similar results were observed in three separate experiments.
