Expression of bomapin, CAP, and β-actin in normal hematopoiesis and in hematological malignancies. RNA was isolated from normal bone marrow (bm; lanes 1-2) and from peripheral blood of normal donors (pb; lanes 3-4) and patients with CLL (lanes 5-6), CML (lanes 7-8), CMML (lanes 9-10), AML (lanes 11-14), and ALL (lanes 15-16). After reverse transcription, bomapin (A) and CAP (B) cDNAs were amplified by PCR for 32 cycles using primer pairs B1 (amplification product, 403 base pairs) and C1 (amplification product, 385 base pairs), respectively. The β-actin (C) cDNA was amplified for 24 cycles (amplification product, 796 base pairs). PCR products (5 μL) were visualized by electrophoresis in a 1.5% agarose gel followed by staining with ethidium bromide. A 1-kb DNA ladder was loaded in the left and right lanes. Bomapin expression was scored to be low or absent in lanes 3-6, 8, 11, and 15-16; medium in lanes 1-2, 7, 9-10, and 12-13; and high in lane 14.