PCR analysis of cell lines derived from bone marrow or peripheral blood of patients with various types of leukemia. The indicated cell lines (CRL 7607, lanes 1-3; CRL 7541, lanes 4-6; KG-1, lanes 7-9; K562, lanes 10-12; THP-1, lanes 13-15; and AML-193, lanes 16-17) were incubated in serum-containing media (10⁶cells/mL, 25 mL/162 cm² flask) in the absence (lanes 1, 4, 7, 10, 13, and 16) or presence of either PMA (10^-8 mol/L, lanes 2, 5, 8, 11, 14, and 17) or TNF-α (30 U/mL; lanes 3, 6, 9, 12, 15, and 18). After 24 hours, the cells were washed by centrifugation and total RNA was isolated, reverse transcribed, and subjected to PCR amplification using primers specific to bomapin (first row, primer pair B2, amplification product: 1,195 base pairs), CAP (second row, primer pair C2, amplification product: 607 base pairs), PAI-2 (third row, amplification product: 897 base pairs), and GAPDH (fourth row, amplification product: 695 base pairs). The PCR products were subjected to electrophoresis in a 1% agarose gel and stained with ethidium bromide. Lanes 19-22 show the PCR products obtained using the aforementioned primers to amplify the vectors containing the cDNAs encoding bomapin (lane 19), CAP (lane 20), PAI-2 (lane 21), or GAPDH (lane 22). Lane 23 contains a 1-kb DNA ladder.