Detection of bomapin transcript in THP-1 cells. THP-1 cells were incubated in the absence (lanes 1 and 4) or presence of PMA (10^-8 mol/L, lanes 2 and 5) or TNF-α (30 U/mL, lanes 3 and 6). RNA was isolated after either 24 hours (lanes 1-3) or 96 hours (lanes 4-6) of treatment and the blot was hybridized (10 μg/lane) to a ³²P-labeled bomapin cDNA probe and exposed for 1 day to radiograph film (A). The blot was rehybridized to a digoxigenin-labeled GAPDH cDNA probe (25 mg/mL) and the bound probe detected by incubation with alkaline phosphatase-labeled antidigoxigenin followed by CDP-Star (Boehringer Mannheim), and exposure to radiograph film for 10 minutes (B).