![Fig. 1. Detection of CBFB-MYH11 fusion transcripts by RT-PCR. (A) Agarose gel electrophoresis of C1-M1 primer set amplification products. (B) Hybridization of PCR products with the inv(16) CBFB internal oligoprobe. In both panels, lane numbers 1 through 6 correspond to samples from PN 14, 13, 22, 1, 18, and 23, respectively (Table 1). Lane 7 represents a relapse sample from PN 23. Lane 8 is a negative control (no RNA). Sample from PN 22 (lane 3) displays a prominent, but slightly smaller PCR product than expected for a type A fusion (∼400 bp in [A]) and does not hybridize with the oligoprobe (B). This PCR product did hybridize positively with a 0.8-kb CBFB cDNA probe (not shown). Lane 4 demonstrates a type D fusion; lanes 6 and 7 show a type C fusion.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/6/10.1182_blood.v91.6.1882/2/blod4064601.jpeg?Expires=1736072939&Signature=37G0fyH0~oaXTpHREeUfjtFF4szDDrT-I07xIdhCyuRURjsyZlf5fyQ6zYxcYy8o8GwQ4MxURW9-xu4GN2ntEQHm8m584KkaEd7SQSNBi7e7H1H8Xi9pEy2Se7bBx7pRn66e~iJSOV84tGmjU8IEKsRQMpRN-32y1RrlOgaTJa3mTUSxmnb7vEXiCN5pWO~p3GTU6Cm12GMUpNXa2K0pDQUfNjDXWr4-00QbUEjSQdNDr8dk8NRVkOwFs-ssx~1d7ti6TnUtcvT9Sp2-VC14vjwoKlHBQxF200G--oB8GhIkH6ekXx1VyNrBk9CZZc5zpMWs1exqdoGDbgJ2LoeKww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of CBFB-MYH11 fusion transcripts by RT-PCR. (A) Agarose gel electrophoresis of C1-M1 primer set amplification products. (B) Hybridization of PCR products with the inv(16) CBFB internal oligoprobe. In both panels, lane numbers 1 through 6 correspond to samples from PN 14, 13, 22, 1, 18, and 23, respectively (Table 1). Lane 7 represents a relapse sample from PN 23. Lane 8 is a negative control (no RNA). Sample from PN 22 (lane 3) displays a prominent, but slightly smaller PCR product than expected for a type A fusion (∼400 bp in [A]) and does not hybridize with the oligoprobe (B). This PCR product did hybridize positively with a 0.8-kb CBFB cDNA probe (not shown). Lane 4 demonstrates a type D fusion; lanes 6 and 7 show a type C fusion.
