Zymosan-induced IL-12 mRNA in WT and ICE-deficient mice. Splenocytes from WT and ICE-deficient mice were incubated for 4 hours with RPMI or 10 μg/mL of zymosan. RT-PCR was performed for the p40 and p35 subunits of IL-12. Minor nonspecific amplicons were noted when p40 was amplified. GAPDH was used as an internal control. Amplification products for p40, p35, and GAPDH for 3 WT and 3 KO mice are shown in (A) (C, RPMI; Z, Zymosan). (B) shows mRNA ratios (mean ± SEM, n = 3).