The effects of F-SC1-Ig on colony assays. A F-SC1-Ig/pEFBOS plasmid or a CD44-Ig/pEFBOS plasmid was transfected into 293T cells. The F-SC1-Ig and the CD44-Ig were purified with a protein A column and added to (A) CFU-IL7, (B) CFU-B, and (C) CFU-c colony assays of mouse BM cells at the indicated concentrations. Results represent mean numbers of colonies per 10⁵ BM cells ± SD (n = 3). Statistically significant differences from control (CD44-Ig) values are indicated by one (P < .05) or two (P < .01) asterisks. Similar results were observed in three independent experiments.