Expession of membrane-anchored SC1/ECM2 on stably transfected NIH3T3 cells. The N- or F-SC1-HT/pEFBOS plasmid or HT/pEFBOS vector was stably transfected into NIH3T3 cells. Each clone was stained with an HPC4 antibody. Shaded histograms depict stainings obtained with the HPC4 antibody, and open histograms represent results obtained with the second antibody alone.