Fig. 1.
![Fig. 1. Effect of different concentrations of TRAP on the platelet surface expression of GPIb and P-selectin. Orgaran anticoagulated whole blood (+ dRGDW) was incubated (5 minutes, 37°C) with various concentrations of TRAP. After 5 minutes, the blood was fixed in 1% paraformaldehyde, incubated with either MoAb 6.20 (against GPIb [▴]) or CD62 MoAb RUU-SP 2.17 (against P-selectin [•]), and analyzed by flow cytometry. Both the binding of the anti-GPIb MoAb before the addition of TRAP and the maximal binding of the anti-CD62 MoAb was assigned 100 arbitrary units of fluorescence. Data represent a typical experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2353/3/blod4074001.jpeg?Expires=1735821950&Signature=h33oE4v0hZpkaM0z25DP~3HeaV7-qkJN~wHztoQ2niDaB1F8gfxbE49iMZXFh7-Cdmfvb1L7YYE3inkHPD1z16z6CKLp9842zcrth9BlKZ3we-K7d66x1LQi-8GyPehkEjBl2ogoJPEUj2r8bBkTKT-fIaTB8QibiUQvszlaPWXYbiIVZ3k42lM9IYr~JUaO2Ok6H5naiPFf3LzH0Is758MnHEG0xclAvnxVlFL2LCcGhsdxi7ggFwoMtNF0dQsuBbCblFuOoJYCDQlvnTbLf21ce8AksJbUzwoNKbuRGavdTj9ODThu3H2vylTU60sYl1-FrwCB~SHvQKJrJApXDA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of different concentrations of TRAP on the platelet surface expression of GPIb and P-selectin. Orgaran anticoagulated whole blood (+ dRGDW) was incubated (5 minutes, 37°C) with various concentrations of TRAP. After 5 minutes, the blood was fixed in 1% paraformaldehyde, incubated with either MoAb 6.20 (against GPIb [▴]) or CD62 MoAb RUU-SP 2.17 (against P-selectin [•]), and analyzed by flow cytometry. Both the binding of the anti-GPIb MoAb before the addition of TRAP and the maximal binding of the anti-CD62 MoAb was assigned 100 arbitrary units of fluorescence. Data represent a typical experiment.
