American Society of Hematology

Fig. 1.

$Fig. 1. Sensitivity of rat exosomal AChE to PI-PLC. (A) Nondenaturing gel electrophoresis. Lane a, detergent extract of rat exosomes; lane b, detergent extract of exosomes treated by PI-PLC as outlined in Materials and Methods. (B) Sedimentation analysis. Samples of exosomal detergent extracts, treated (○) or not (•, ▵) by PI-PLC, were layered on 5% to 20% sucrose gradients containing (•) or not (○, ▵) 1% Triton X-100, and centrifuged for 19 hours at 36,000g. Collected fractions were assayed for AChE activity as described in Materials and Methods. The vertical arrows indicate the positions of E coli β-galactosidase (16S) and alkaline phosphatase (6.1S).$

Sensitivity of rat exosomal AChE to PI-PLC. (A) Nondenaturing gel electrophoresis. Lane a, detergent extract of rat exosomes; lane b, detergent extract of exosomes treated by PI-PLC as outlined in Materials and Methods. (B) Sedimentation analysis. Samples of exosomal detergent extracts, treated (○) or not (•, ▵) by PI-PLC, were layered on 5% to 20% sucrose gradients containing (•) or not (○, ▵) 1% Triton X-100, and centrifuged for 19 hours at 36,000g. Collected fractions were assayed for AChE activity as described in Materials and Methods. The vertical arrows indicate the positions of E coli β-galactosidase (16S) and alkaline phosphatase (6.1S).

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