Binding of G-CSF to K pneumoniae B5055 whole cell lysates as assessed by immunoblotting. L, bacterial cell lysate; PK-L, bacterial cell lysate pretreated with proteinase K; +/p, G-CSF preabsorbed with rabbit anti-human G-CSF-IgG. Lanes 1 through 5 are from a representative blot. Lanes 6 through 8 are from a silver-stained identical replica of the gel. Binding of G-CSF to specific bands is indicated by arrows; molecular weight (in kD) is indicated on the left.