SCF mRNA RT-PCR analysis. The specific 409- and 494-bp PCR fragments are visible in 5 out of 6 (83%) of sarcoma cell lines. Lane 1, 6647 (ES); lane 2, TC32 (PNET); lane 3, TC106 (ES); lane 4, PDN13 (PNET); lane 5, PDN12 (PNET); lane 6, PDE02 (ES); lane 7, B lymphoblastoid EBV-transformed cell line SCF mRNA neg; lane 8, No DNA.