Fig. 6.
Effect of cystatin on thrombin-stimulated plasminogen activation (A) and on the reactivation of platelet u-PA (B). (A) Platelets (3.5 × 108/mL) were incubated at 37°C with plasminogen, S2403, and thrombin at 2.5 U/mL (circles), 5 U/mL (triangles), or 10 U/mL (squares) in the absence (open symbols) or presence of 20 nmol/L cystatin (closed symbols) and assayed for plasmin activity. (B) Zymogram of the platelet incubation mixtures containing thrombin at 0,1,5 2.5 u/mL,2,6 5 u/mL,3,7 10 u/mL4,8 after 1, 3, or 5 hours of incubation in the absence 1-4 or presence5-8 of cystatin.
