The H2K-BCL-2 transgene protects cells from apoptosis. (A) Survival of unstimulated splenocytes from H2K-BCL-2 transgenic mice (thick lines) and wild-type littermates (thin lines) in vitro. The transgenic mice were from founderlines 1038, 1039, and 1053. Data from five animals per group, cultured in four separate experiments. The FACS plots show the B (B220⁺) and T (CD3⁺) cell populations in transgenic spleen at day 0, 20, and 62 of culture and in the wild-type spleen at day 9. (B) Survival of bone marrow-derived mast cells in vitro from H2K-BCL-2/1043 transgenic mice (thick lines) and wild-type littermates (thin lines) after IL-3 withdrawal. The cell culture results from two animals per group are depicted.