MoAb90 induces apoptosis of activated T lymphocytes. (A) DNA fragmentation: PBL were incubated 3 days with medium alone (lanes 1 through 3) or PHA (5 μg/mL) (lanes 4 through 6). Dead cells were removed and viable cells were treated for 24 hours with control IgG1 (lanes 1 and 4), W6/32 (lanes 2 and 5), or MoAb90 (lanes 3 and 6) at 10 μg/mL. (B through D) Morphology of 3-day PHA-activated PBL after Hoescht 33342 staining. After 3 days of culture, PHA-activated PBL were obtained. Dead cells were removed and viable cells (106/mL) were incubated in 96-well microplates in the presence of the MoAbs. Morphology was analyzed by fluorescence microscopy. (E through G) Electronic microscopy of 3-day PHA-activated PBL. PBL were cultured during 3 days with PHA (5 μg/mL). Viable cells were then treated for 24 hours with control IgG1 (10 μg/mL) (B and E), W6/32 (10 μg/mL) (C and F ), or MoAb90 (10 μg/mL) (D and G).
